RDO Procedures & Instructions

RDO Rapid Decalcifier procedures

RDO Rapid Decalcifier procedures

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DECALCIFICATION TIME CHART

Bone Type
Mouse
Hamster
Rat
Rabbit
Monkey
Dog
Turbinates
Whole Heads 4 hours, check
8-10 Hours
8-10 Hours
12...
12...
12...
Femur
45 minutes
2 Hours
2-3 Hours
8-10 Hours
12...
12...
Sternum
35-45 Minutes
1 Hour
1 Hour
6-8 Hours
6-8 Hours
6-8 Hours
Rib
45 Minutes
1-2 Hours
1-2 Hours
4-6 Hours
6 Hours
6 Hours
Vertebrae
1 Hour
2 Hours
2 Hours
6 Hours
6 Hours
6 Hours

All tissues should be checked at least halfway through the time given.
12... = Check regularly for desired endpoint
Decalcification times prepared by Mary Parker, Durham, NC.

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