Technical

Technical Information RDO Rapid Decalcifier

Both RDO and RDO Gold are unsurpassed decalicfiers that can be tailored to suit your specific lab routine. As with most acids, nuclei acids in the cell can become subject to ribonuclease digestion, resulting in a loss of basophilic properties. Careful monitoring should be used with RDO to avoid over decalcification. Decalcification will depend on the thickness and density of specimens. Overnight decalcification should be avoided. Full Strength RDO is used for hard compact bone, ie. femur heads. Both RDO and RDO Gold may be diluted with tap water to permit multipurpose use. Dilution with tap water is recommended for mildly calcified specimens such as bone marrow biopsies (core). In either case, the standard procedure should be to check the specimen periodically to determine the endpoint of decalcification.

 

Technical Procedures & Instructions for RDO Rapid Decalcifier

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Technical Procedures & Instructions for RDO GOLD

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Technical Reprints

DECALCIFICATION TIME CHART

Bone Type
Mouse
Hamster
Rat
Rabbit
Monkey
Dog
Turbinates
Whole Heads 4 hours, check
8-10 Hours
8-10 Hours
12...
12...
12...
Femur
45 minutes
2 Hours
2-3 Hours
8-10 Hours
12...
12...
Sternum
35-45 Minutes
1 Hour
1 Hour
6-8 Hours
6-8 Hours
6-8 Hours
Rib
45 Minutes
1-2 Hours
1-2 Hours
4-6 Hours
6 Hours
6 Hours
Vertebrae
1 Hour
2 Hours
2 Hours
6 Hours
6 Hours
6 Hours

All tissues should be checked at least halfway through the time given.
12... = Check regularly for desired endpoint
Decalcification times prepared by Mary Parker, Durham, NC.